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. Author manuscript; available in PMC: 2019 Dec 5.
Published in final edited form as: Sci Transl Med. 2019 Jun 5;11(495):eaav5597. doi: 10.1126/scitranslmed.aav5597

Fig. 7. Lymphocyte deficiency ameliorates airway neutrophil accumulation and lung tissue injury in Aire−/− mice.

Fig. 7.

(A) Representative images of H&E staining of Aire+/+,Aire−/−,Aire−/−Tcra−/−, and Aire−/−lghm−/− mouse lung and of immunohistochemical staining with the lymphocyte markers CD3 and B220 in Aire−/− mouse lung. Original magnification, 40×. Scale bars, 200 μm. n = 6 to 10 per group; one to three independent experiments. (B) Neutrophils in the BAL. Percentage of neutrophils within total CD45+ leukocytes (top; *P < 0.05) and total number of neutrophils per lavage (bottom; **P< 0.01 and ***P< 0.001). n = 7 to 10 per group; three to four independent experiments. (C) Lymphocyte populations in lung tissue. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. n = 7 to 10 per group; three independent experiments. (D) Lung histology scores. ****P < 0.0001. n = 6 to 10 per group; three independent experiments. Differences between groups were determined using one-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons. Quantitative data represent means ±SEM.