Fig. 3.

The effect of WIN55,212-2 on the startle response after a dark challenge. The behavior of the larvae during the first 5 s of the dark challenge was analyzed. a Percentage of larvae responding to the dark challenge by showing increased swimming velocity. From 125 nM and higher, a strong decrease of responsive fish can be noticed. b From the responsive fish, the reaction time was calculated. The latency was strongly reduced at concentrations of 125 nM and higher. Group sizes are reported in parentheses. Data shown are means ± SEM. Significant differences compared to the corresponding vehicle-treated control group are indicated. ***P ≤ 0.001; ****P ≤ 0.0001