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. 2019 Jul 22;17:80. doi: 10.1186/s12964-019-0395-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Analysis of the effect of γ-Secretase inhibitor, DAPT on PC3 cells. a. Equal amounts of total lysates (40μg) were immunoblotted for CD44-ICD. IB analysis of PC3 cells untreated, PC3 cells treated with DMSO control, PC3 cells treated with increasing concentrations (1 μM - 20 μM) γ-secretase inhibitor (DAPT) lanes 3–7, LNCaP cells, and PC3 cells knockdown of CD44 was immunoblotted with an antibody to CD44-ICD (top panel) and GAPDH (bottom panel). b. PC3 cells treated with DMSO control (lane 1), 1 μM of DAPT (lane 2) and 5 μM of DAPT (lane 3) was immunoblotted with an antibody to RUNX2 (top panel), CD44-ICD (middle panel) and GAPDH (bottom panel). Two asterisks (**) represents the 25 kDa CD44 extracellular truncation (CD44-EXT) while one asterisk (*) represents the 20 kDa CD44 extracellular truncation (CD44-EXT)