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. 2019 Jul 22;17:80. doi: 10.1186/s12964-019-0395-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — The effect of RUNX2 overexpression on the expression of metastasis-related genes. a-e. The real-time PCR analysis of OCT4, SOX2, CD44, OPN, and MMP9 expression in PC3, PC3 cells transfected with RUNX2, and LNCaP cells. f. An equal amount of protein lysates (30 μg) made from PC3 (lane 1) and PC3 cells transfected with RUNX2 (lane 2) were immunoblotted with Ezrin, OPN, RUNX2, SOX2, MMP9 antibodies to detect total cellular levels of the respective proteins. GAPDH was used as a loading control for real-time PCR and IB analysis (A-G). The results represent one of three separate experiments performed with the same results