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. 2019 Jul 22;19:189. doi: 10.1186/s12935-019-0876-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The effect of VEDT on cell death and survival. a Time course of VEDT activity on decoy receptors and death receptor pathway (DR4, DR5, DCR1, DCR2, DCR3) and c-FLIP_s in panel of pancreatic cancer cells by Western blot (n = 3). b Effect of transient ectopic transfection of c-FLIP_s in pCMV6-AC-GFP plasmid vector on apoptosis in MiaPaCa-2 cells treated with VEDT for 24 h as measured by TUNEL and quantified by analytic microscopy (inset). Columns, means; bars, standard deviation (n = 3, **P < .01 and ***P < .001). c Effect of VEDT on caspase-8 enzymatic activity in Mia-GFP and Mia-FLIP cells. Columns, means; bars, standard deviation (n = 3, *P < .001 and ***P < .05). d Effect of VEDT on survival in Mia-GFP and Mia-FLIP cells. Columns, means; bars, standard deviation (n = 3, *P < .001, **P < .01, and ***P < .05)