Fig. 2.
HERG1 stimulates proliferation of ESCC cells. a Colony formation was enhanced in HERG1-overexpressing TE-1 cells and reduced in HERG1-silenced KYSE-30 cells (n = 3). b In MTT assays, cell viability was increased by HERG1 overexpression and decreased by HERG1 silencing (n = 3). c Equal cell densities (1 × 103) with different HERG1 expression were plated into 12-well plates, and the cells were counted at 0, 24, 48, 72, and 96 h (n = 3). d BrdU assays showed increased DNA synthesis in HERG1-overexpressing TE-1 cells, and decreased DNA synthesis in HERG1-silenced KYSE-30 cells (n = 3). e HERG1-overexpressing cells proceeded faster than control cells into the S phase, whereas HERG1-silenced cells were arrested in the G1 phase (n = 3). (f and g) mRNA and protein levels of cell cycle-related molecules p21 and cyclin D1 in different conditions were analyzed by qPCR (f) and western blotting (g); n = 3. (h and i) Cell apoptosis was evaluated by flow cytometry (h) and TUNEL assays (i) in TE-1 cells and KYSE-30 cells with different HERG1 expression (n = 3). *: p < 0.05