Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 29;597(11):2867–2885. doi: 10.1113/JP277650

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

A and B, Stimulus‐induced Ca²⁺ spikes from experiments shown in Figs 4 and 6 were aligned to the rising phase of the Ca²⁺ transient. Traces are averaged Ca²⁺ transients induced by (A) electrical stimulation () or (B) vasopressin stimulation followed by vasopressin plus electrical stimulation (); n = 16–41 cells per condition. Bars above traces indicate the duration of the stimulus and broken line indicates a gap in time series. Summary data showing (C) Ca²⁺ spike amplitude and (D) spike width calculated at half‐height. Ca²⁺ spikes were induced by electrical stimulation (ES; 4 V, 4 Hz), perfusion with 10 pm vasopressin (VP) or vasopressin plus electrical stimulation (VP + ES). E, percentage of the field responding with an increase in [Ca²⁺]_c that occurred within 5 min after commencing the indicated stimulus. Scatter plots are the mean ± SD with each symbol representing data from a separate liver, n = 6–10 liver preparations and 150–300 hepatocytes analysed per stimulus. The blue, green and purple symbols show data from the same liver preparation put through all three sequential stimuli. *Significantly different compared to ES, ^**Significantly different compared to ES or VP; P < 0.05; one‐way ANOVA with a post hoc Bonferroni test.

Inline graphic — A and B, Stimulus‐induced Ca²⁺ spikes from experiments shown in Figs 4 and 6 were aligned to the rising phase of the Ca²⁺ transient. Traces are averaged Ca²⁺ transients induced by (A) electrical stimulation () or (B) vasopressin stimulation followed by vasopressin plus electrical stimulation (); n = 16–41 cells per condition. Bars above traces indicate the duration of the stimulus and broken line indicates a gap in time series. Summary data showing (C) Ca²⁺ spike amplitude and (D) spike width calculated at half‐height. Ca²⁺ spikes were induced by electrical stimulation (ES; 4 V, 4 Hz), perfusion with 10 pm vasopressin (VP) or vasopressin plus electrical stimulation (VP + ES). E, percentage of the field responding with an increase in [Ca²⁺]_c that occurred within 5 min after commencing the indicated stimulus. Scatter plots are the mean ± SD with each symbol representing data from a separate liver, n = 6–10 liver preparations and 150–300 hepatocytes analysed per stimulus. The blue, green and purple symbols show data from the same liver preparation put through all three sequential stimuli. *Significantly different compared to ES, ^**Significantly different compared to ES or VP; P < 0.05; one‐way ANOVA with a post hoc Bonferroni test.