Fig. 2.

Prenatal BP-3 diminished the expression of autophagy-related factors in mouse embryonic neurons. The effect of prenatal BP-3 on the mRNA expression levels of Becn1, Nup62, Atg7, Map1lc3a, and Map1lc3b (a) in neocortical cultures. b–e The effects of prenatally administered BP-3 on the protein levels of autophagy-related factors (BECLIN-1, NUP62, MAP1LC3A, and MAP1LC3B). The concentrations of the protein were measured using specific ELISA and are presented as a percentage of the control (b) and as pg of specific protein per μg of total protein (c). For the western blot analyses, protein samples were denatured, electrophoretically separated, transferred to PVDF membranes, and subjected to immunolabeling (d). The relative protein levels of BECLIN-1, NUP62, ATG 7, MAP1LC3A, and MAP1LC3B were presented as a percentage of the control (e). Each bar represents the mean of three independent experiments ± SE. The number of replicates in each experiment ranged from 2 to 3. *p < 0.05, **p < 0.01, and ***p < 0.001 versus control cultures