Fig. 1.

Foxg1^cre/+ adult hippocampus expresses altered miRNA levels of miR200b/a/429 and Htr2c families. a Representative immunoblot and quantification of FOXG1 protein levels in Foxg1^cre/+ and WT hippocampus show that FOXG1 protein is reduced by 60% in Foxg1^cre/+ compared to control levels (dashed line). Mean with SEM, ***p < 0.001, one-sample Student’s t test. n = 6. b Volcano plot of small RNA-Seq of Foxg1^cre/+ compared to WT hippocampus indicates that expression of miR200b/a/429 family is significantly decreased, whereas miRNAs from Htr2c gene significantly increased (dashed lines: DEseq2 p-adjusted value = 0.05 (black); FC = ± 2.0 (green); FC = ± 1.5 (red)). Values for adjusted p value were plotted on the y-axis. n = 9. c qRT-PCR validation confirms significantly altered expression levels of miRNAs of miR200b/a/429 and Htr2c families in 6-week-old hippocampus of Foxg1^cre/+ animals. Mean with SEM, *p < 0.05, **p < 0.01, unpaired Student’s t test. n = 3. d Expression of precursor transcripts of miR200b/a/429 in Foxg1^cre/+ hippocampus using qRT-PCR reveals decreased expression of pre-miR200b and pre-miR200a, while expression of pre-miR429 was not detectable (n.d.). Mean with SEM, *p < 0.05, **p < 0.01, unpaired Student’s t test. n = 3