Skip to main content
. 2018 Dec 11;56(7):5188–5201. doi: 10.1007/s12035-018-1444-7

Fig. 3.

Fig. 3

miR200b/a/429 overexpression reduces PRKAR2B protein levels. a Immunoblots of WT and Foxg1cre/+ hippocampus using anti-PRKAR2B antibodies show increased expression in the adult hippocampus of Foxg1cre/+ animals. n = 7. b Immunoblots of N2a cells overexpressing miR200 family or miR200 sponge plasmids. Overexpression of miR200 family reduces PRKAR2B levels compared to miR200 sponge-transfected N2a cells. n = 3. c Densitometric quantification of a and b. Reduced levels of FOXG1 result in increased levels of PRKAR2B in the adult hippocampus. miR200 family member overexpression reduces PRKAR2B levels significantly when compared to miR200 sponge (dashed line represents control expression levels). Mean with SEM, *p < 0.05, **p < 0.01, one-sample Student’s t test. n = 7. Foxg1cre/+ hippocampus, and n = 3 for miR200 OE. d Results of luciferase assays to detect degradation of a transcript containing a wild type (WT), inverted or T7 seed sequence in the Prakr2b-3′UTR. Overexpression of miR200b/a/429 degrades transcripts with WT-seed but not with inverted or T7 seed in the Prkar2b-3′UTR, when compared to control vector expression (dashed line). Mean with SEM, **p < 0.01, one-sample Student’s t test. n = 3–5