Fig. 4.

FOXG1 suppresses Prkar2b expression through direct and indirect mechanisms. a UCSC Genome browser view of Prkar2b gene region indicating FOXG1 binding site (FOXG1 ChIP peak, shown in green) from FOXG1 adult hippocampus ChIP-Seq data. b UCSC genome browser view of miR200b/a/429 gene showing the cloned region in the black bar, which does not include promoter, 5′ and 3′UTR regions. No FOXG1 binding sites were predicted in the miR200b/a/429 gene. c Transcript levels of Prkar2b are reduced after FOXG1-FL and FOXG1-D2 expression in N2a cells compared to empty vector-transfected cells. Mean with SEM, *p < 0.05, **p < 0.01. n = 5. d Results of luciferase assays to detect transcriptional influence of FOXG1 on the region around the peak enriched after FOXG1–ChIP-Seq in the 5′ region of the Prkar2b gene. FOXG1 suppresses luciferase activity significantly, when compared to control. Mean with SEM, **p < 0.01, unpaired Student’s t test. n = 5