Fig. 5.

Mass spectrometry and Co-IP reveal interaction of FOXG1 with DDX5 and the microprocessor. a Scatterplot of the FOXG1 interactome highlights its interaction with DDX5. The fold enrichment of two independent FOXG1-Au1 co-IP replicates of the MS analysis are plotted on the x- and y-axis, respectively, and detected proteins are colour coded with regard to the number of individual peptides mapping into the identified protein. n = 2. b DAVID KEGG pathway analysis with the most significant pathways. p values < 0.049. c Representative immunoblot after FOXG1-HA co-IP using anti-HA, anti-DDX5, anti-DROSHA and anti-DGCR8 shows that DDX5 and the microprocessor proteins interact with FOXG1 in N2a cells. Overexpression of FOXG1-Au1 serves as control for specificity of the HA-co-IP. d Representative immunoblot using anti-FOXG1, anti-DDX5, anti-DROSHA and anti-DGCR8 antibodies after co-IP of endogenous FOXG1 and DDX5, respectively, from protein extracts of adult mouse hippocampus. n = 3