Fig. 8.

Increased levels of Foxg1 and Ddx5 KD decrease miR200b expression in primary hippocampal neurons. a qRT-PCR of Ddx5 and Foxg1 in Foxg1 KD primary hippocampal cells, Foxg1 KD increases expression of Ddx5.b qRT-PCR of mature miR200 family expression after KD of Foxg1. Expression levels of mature miRNA200 family do not change. c qRT-PCR of Ddx5 and Foxg1 in Ddx5 KD primary hippocampal cells. Ddx5 KD results in increased expression of Foxg1.d qRT-PCR of mature miR200 family expression after KD of Ddx5. Reduced expression of Ddx5 with concomitant increase in Foxg1 expression causes significant reduction of mature miR200b expression in primary hippocampal neurons. All data are represented as mean with SEM, *p < 0.05, **p < 0.01, one-sample Student’s t test. n = 3