Fig. 2.

Cytosolic TDP-43 accumulations in double-transgenic mice. a Cytosolic, nuclear, and total protein extracts from the spinal cord of non-transgenic (NTG), UBQLN2^P497H (UBQ), TDP-43^G348C (TDP), and double-transgenic UBQLN2^P497H; TDP-43^G348C (UBQ; TDP) mice (n = 3, age = 5 months). b Cytosolic, nuclear, and total protein extracts from the brain (n = 3, age = 5 months). c Cytosolic fraction from the brain of TDP-43^G348C and double-transgenic mice at 5 months and 8 months of age (n = 3). d Quantification of TDP-43 levels vs GAPDH levels in the cytosolic fractions of the brain at 5 and 8 months of age. e Quantification of the TDP-43 levels vs GAPDH levels in the cytosolic fraction of spinal cord (n = 3, age = 5 months). f Quantification of the TDP-43 levels vs GAPDH levels in the cytosolic fraction of brain (n = 3, age = 5 months). g Quantification of the TDP-43 levels vs P84 nuclear matrix levels in the nuclear fraction of the spinal cord (n = 3, age = 5 months). h Quantification of the TDP-43 levels vs P84 nuclear matrix levels in the nuclear fraction of the brain (n = 3, age = 5 months)