Fig. 1.

Possible associations with clinicopathological features. a Profile of detected variants showed overall low level of heteroplasmy and hierarchical cluster analysis could not discriminate pituitary adenoma samples based on either histological type, Ki-67 index, or recurrent/non-recurrent status. The colour scale indicates the ratio of heteroplasmy obtained by NGS, where 0 (blue) shows 100% reference allele and 1 (green) shows 100% variant allele. b Number of the found variants in pituitary adenoma samples, grouped by histological type and ranked from fewer to more variants. Analysing the samples harbouring the highest number of variants obtained that they have the highest Ki-67 indices independently of histological type. c Regarding the number of the variants, we identified 143, 58, and 52 unique variants appeared only in GO, HN, and GH-secreting adenomas, respectively. Legends: rec: recurrent, non-rec: non-recurrent, Ki-67 group 1: Ki-67 proliferation index is between 1 and 4%, Ki-67 group 2: Ki-67 proliferation index is between 5 and 10%, Hist histological subtype, GH growth hormone producing, GO gonadotroph, HN hormone immunonegative