Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 27;377(2):259–268. doi: 10.1007/s00441-019-03017-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

PMC Copyright notice

Fig. 5 — The expressions of MrgprD in murine macrophage-like RAW 264.7 and human T lymphocyte Jurkat cell lines. (a, b) The expressions of MrgprD in RAW 264.7 and Jurkat cells were determined by RT-PCR (a) and Western blotting (b). The tissue lysate of liver was used as the negative control and the lysate of DRGs was the positive control. (c–c″ and d–d″) Confocal images showed the presence of MrgprD protein (red) in RAW 264.7 (c–c″) and Jurkat cells (d–d″). Scale bars 20 μm. (e) The cytoplasmic/nuclear fractions were separately extracted from Raw 264.7 and Jurkat cells (in triplicates) and analyzed for the expressions of MrgprD, Lamin B and GAPDH by Western blotting. The expressions of MrgprD and GAPDH but not Lamin B, in cytoplasmic fractions were detected (upper panels). By contract, MrgprD was not expressed in the nuclear fractions of Raw 264.7 and Jurkat cells. The purity of nuclear fraction was determined by the expression of Lamin B and the absence of GAPDH (lower panels)