Table 2.

Comparison of the Staphylococcus aureus SI and CI for One‐ and Two‐Step Permeabilization FISH Methods Using a STAAUR Oligo

Treatment	SIa	CIb
One‐step FISH assay with oligo‐fc	23.44	0.55
One‐step FISH assay with oligo‐f and Tween 20® d	24.85	0.71
One‐step FISH assay with oligo‐bc	17.19	0.19
Two‐step FISH assay with oligo‐bc	23.27	0.48
Two‐step FISH assay with oligo‐fe	27.31	0.45

SI, signal intensity; CI, confidence interval; FISH, fluorescence in situ hybridization. Exposure time was the same for each image acquisition. The results were consistent across the ten isolates tested.

^{a a}Mean signal intensity in 8‐bit Gray‐scale.

^{b b}Confidence interval was calculated at 95%.

^{c c}As described in Table 1.

^{d d}The one‐step FISH assay with a 5‐min, 1% Tween 20^® step at room temperature before hybridization.

^{e e}The two‐step FISH assay using a oligo‐f, without the streptavidin incubation step.