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. 2011 Jan 19;25(1):52–60. doi: 10.1002/jcla.20430

Table 1.

Assay Conditions and Instrument Parameters for Serum Protein Measurement on the cobas c 501

α1AT α2M Alb Apo AI Apo B C3 C4 Hpt IgA IgG IgM Oro Tf Ttr
Antiserum dilutiona 1:7 1:10 1:7 1:6 1:5 1:15 1:10 1:10 1:10 1:15 1:10 1:7 1:15 1:8
Number of tests/cassette 178 200 178 178 190 190 190 172 190 190 190 190 200 198
Fill volume (ml)
 R1, position A 25.65 25.65 25.65 25.65 25.65 25.65 25.65 25.65 25.65 25.65 25.65 25.65 25.65 25.65
 R2, position B 7.7 7.3 7.7 6.8 6.7 6.7 6.7 6.2 7.7 6.5 7.7 7.7 6.4 7.7
Instrument settings
Assay type 2 point end
Wavelength (2nd/1st) 700 nm/340 nm
Reaction time (min) 10 6 6 6 6 6 6 6 6 6 6 10 6 10
Assay points (initial) 10 10 10 10 10 10 10 10 10 10 10 10 10 10
(final) 70 42 42 42 42 42 42 42 42 42 42 70 42 70
Reaction direction Increasing
Sample dilutionb 1:21
 Sample volume (µl)
  Normal 3.0 9.3 graphic file with name JCLA-25-52-e004.jpg 3.0 12.6 10.5 21.0 4.0 4.6 2.1 10.5 5.5 3.7 20.2
  Decrease Inline graphic c 5.6 graphic file with name JCLA-25-52-e006.jpg graphic file with name JCLA-25-52-e005.jpg graphic file with name JCLA-25-52-e008.jpg 6.3 8.4 graphic file with name JCLA-25-52-e007.jpg graphic file with name JCLA-25-52-e003.jpg graphic file with name JCLA-25-52-e001.jpg 2.1 2.9 3.7 11.2
  Increase 14.9 18.7 graphic file with name JCLA-25-52-e002.jpg 6.0 21.0 21.0 35.0 33.6 32.2 21.6 31.5 10.5 7.5 35.0
Reagent bottle setting
 Reagent volume (µl)d
  R1, position A 112 100 112 112 105 105 105 116 105 105 105 105 100 101
  R2, position B 28 23 28 23 21 21 21 21 26 20 26 26 20 25
Calibration mode RCM RCM2T1 RCM RCM RCM RCM2T1 RCM2T1 RCM2T1 RCM RCM RCM RCM RCM2T1 RCM2T1
Point 6 6 5 6 6 6 6 6 6 6 6 6 6 6
Span point 6 6 5 6 6 6 6 6 6 6 6 6 6 6
Calibration curve Concentrations are expressed in g/l (actual mass values are lot specific)
Standard 1 0.36 0.56 10.88 0.38 0.22 0.38 0.08 0.32 0.49 2.62 0.25 0.42 0.74 0.06
Standard 2 0.71 1.13 21.76 0.85 0.49 0.76 0.16 0.65 0.98 5.24 0.49 0.64 1.48 0.13
Standard 3 1.07 1.69 32.64 1.12 0.64 1.14 0.23 0.97 1.47 7.86 0.74 1.06 2.22 0.19
Standard 4 1.79 2.82 54.40 1.66 0.95 1.91 0.39 1.62 2.45 13.11 1.24 1.59 3.71 0.32
Standard 5 2.68 4.22 81.60 2.58 1.48 2.86 0.58 2.42 3.68 19.66 1.85 2.12 5.56 0.48
Standard 6 3.57 5.63 − 3.17 1.82 3.81 0.78 3.23 4.90 26.21 2.47 3.48e 7.41 0.64
a

aAntiserum is diluted in 0.2 M Tris HCl (see text for details).

b, c

bAssay controls and specimens are diluted 1:21 in PBS with the exception of apos AI and B which are diluted 1:21 in Diluent A.cIn this example, the analyzer delivers 3 µl of sample from a 1:5 dilution (20+80 µl).

d

dR1 is reaction buffer; R2 is diluted antiserum.

e

eStandard 6 for Oro is a 1:13 dilution of the neat calibrator 1.