Table 2.
Result of Methodological Evaluation
| Intraassay and interassay imprecisiona (CV) (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Low | High | Verify reference interval | |||||||
| Method | Mean** (mg/l) | Intra | Inter | Mean** (mg/l) | Intra | Inter | Linearity (R) | Recoverya (%) | (mg/l) (%)c |
| Nephelometry | 12.7 | 3.0 | 2.2 | 165 | 2.1 | 1.3 | 1.00 | 99.6 | 2.4–18.6 (100) |
| Turbidimetry | 15.8 | 5.7 | 4.9 | 187.4 | 4.3 | 3.2 | 0.95 | 97.8 | 4.8–32.7 (90) |
| Colloidal gold method | 18 | 2.9 | 2.0 | 186 | 3.3 | 1.5 | 0.99 | 96.3 | (<5)−15 (100) |
| RIA | 14.8 | 7.8 | 8.6 | 173.6 | 6.1 | 3.4 | 0.99 | 99.8 | 2.8–21.1 (95) |
| CLIA | 20.1 | 5.8 | 3.2 | 208.2 | 5.5 | 3.1 | 0.98 | 101.6 | 5.7–37.4 (80) |
RIA, radioimmunoassay; CLIA, chemiluminescence immunoassay.
a
aIntraassay and interassay imprecision CV, and recovery were represented as average.
bThese values represent the mean value of samples in intraassay imprecision by different methods.
c
c“%”means what the percentage of urinary albumin concentration from 20 healthy people were less than 20 mg /l.