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. 2011 May 12;25(3):166–173. doi: 10.1002/jcla.20451

Figure 2.

Figure 2

Effect of fixation, autolysis, and their interaction on relative quantification in DLD‐1 and Colo320 cells showed a factorial experimental design for DLD‐1 and Colo320 cells, the main effect of fixation, autolysis period, and their interaction on the relative quantification of HNRPM against β2‐microglobulin were all investigated. Meanwhile, Hexamer and Cocktail PCR primer were used respectively. The ANOVA results showed no statistical significance in relative quantification of HNRPM among different fixation times, autolysis delay, and the combination of both.