Table 3.
Coefficient of Variation (CV) Calculated for C T Value With Different Fragment and Formalin Fixation in FFPE Tissue (Hexamer and Cocktail PCR primer)
| C T value (hexamer) | C T value (cocktail PCR primer) | ||||||
|---|---|---|---|---|---|---|---|
| Gene/amplicon | Fixation period | n | Mean (arbitrary unit) | CV (%) | n | Mean (arbitrary unit) | CV (%) |
| β2‐Microglobulin 98 bp | Fresh | 3 | 18.319 | 0.328 | 4 | 19.662 | 15.80 |
| 3 hr | 3 | 23.075 | 8.093 | 8 | 24.127 | 13.00 | |
| 3 days | 3 | 25.302 | 6.881 | 8 | 24.550 | 15.09 | |
| β‐actin 265 bp | Fresh | 3 | 17.500 | 3.543 | 4 | 18.724 | 15.18 |
| 3 hr | 3 | 24.026 | 14.360 | 8 | 22.726 | 22.31 | |
| 3 days | 3 | 28.808 | 23.187 | 8 | 25.252 | 21.87 | |
| RQ of β‐actin/β2‐microglobulin | Fresh | 3 | 1.047 | 0.382 | 4 | 1.052 | 10.31 |
| 3 hr | 3 | 0.873 | 6.758 | 8 | 1.0716 | 13.40 | |
| 3 days | 3 | 0.902 | 4.213 | 8 | 0.995 | 11.58 | |