Fig. 2.

Ctr1c can bind both copper binding domains of Ccs. a Nickel pulldown assays were loaded into a clear-bottom 384-well plate and visualized fluorescently using the Typhoon 9500. Lane 1: Input, Lane 2: Flow-through, Lanes 3-5: Washes, Lane 6: Elution, Lane 7: Buffer blank. b This graph of fluorescence intensity is normalized to mutant Ccs lacking copper binding motifs. (c, Top) Lane 1: Sod1 stripped of metals, Lane 2: Sod1 with Cu(I) − Ctr1c, Lane 3: Sod1 with apo-MxAxxA Ccs, Lane 4: Sod1 with apo-MxAxxA Ccs and Cu(I)-Ctr1c, Lane 5: Sod1 with Cu(1)- WT Ccs. (C, Bottom) Lane 1: Sod1 stripped of metals, Lane 2: Sod1 with Cu(I)-Ctr1c, Lane 3: Sod1 with apo-AxA Ccs, Lane 4: Sod1 with apo-AxA Ccs and Cu(I)-Ctr1c, Lane 5: Sod1 with Cu(1)-WT Ccs