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. 2019 Jul 10;32(4):695–705. doi: 10.1007/s10534-019-00206-3

Fig. 3.

Fig. 3

Immature Sod1 can form a stable Sod1·Ccs·Ctr1c heterotrimeric complex. Lane 1: beads supernatant, Lane 2: Cu(I)-Ctr1c488 supernatant, Lane 3: His8-Ccs supernatant, Lane 4: Sod1 supernatant, Lane 5: His8-Ccs·Sod1 supernatant, Lane 6: His8-Ccs·Sod1 and Cu(I)-Ctr1c488 supernatant, Lane 7: beads elution, Lane 8: Cu(I)-Ctr1c488 elution, Lane 9: His8-Ccs elution, Lane 10: Sod1 elution, Lane 11: His8-Ccs·Sod1 elution, Lane 12: His8-Ccs·Sod1 and Cu(I)-Ctr1c488 elution. The right image is the same gel as the left (stain free), but visualized fluorescently