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. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: Theriogenology. 2018 Dec 24;127:41–48. doi: 10.1016/j.theriogenology.2018.12.031

Fig. 1.

Fig. 1.

Characteristics of bovine eggs obtained through in vitro maturation (IVM). (a) The percent of bovine oocytes at each stage of meiosis (scored by chromatin configuration) following specific times of IVM. (b) Average diameter of bovine eggs at different meiotic stages following IVM (**, p = 0.0037; ***, p < 0.0001; ns, not significant). (c) Bovine eggs arrested at metaphase of meiosis II were fixed and stained for actin, tubulin, and DNA to assess cytoskeleton morphology (Scale bars, 20 μm). A representative image is shown, and the meiotic MII spindle is highlighted in the inset. The asterisk marks the first polar body.