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. Author manuscript; available in PMC: 2019 Jul 23.
Published in final edited form as: Biochem J. 2019 Mar 12;476(5):859–873. doi: 10.1042/BCJ20180916

Figure 6. GRN-3 promotes aggregation of oligomeric Aβ42.

Figure 6

Western blot analysis of the effect of GRN-3 (A) or rGRN-3 (B) on Aβ42 oligomers (LFAOs). Aβ42 monomer control (20 μM; lanes 1 and 2). LFAOs alone (3.0 μM; lanes 3 and 4) or after incubation with Aβ42 monomer for 72 h at 25°C (20 μM; lanes 5 and 6). The co-incubation of LFAOs (3.0 μM) and GRN-3 or rGRN-3 (40 μM) in the absence of Aβ42 monomer (lanes 7 and 8), and co-incubation of similar concentrations of LFAOs and GRN-3 or rGRN-3 in the presence of Aβ42 monomer (20 μM; lanes 9 and 10). For lanes 9 and10, GRN-3 (or rGRN-3) and LFAOs were incubated for 1 h prior to the addition of Aβ42 monomers. In all lanes, T represents the total sample while S denotes the supernatant after centrifugation for 20 min at 18 000×g.