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. Author manuscript; available in PMC: 2019 Jul 23.
Published in final edited form as: AAPS J. 2019 Feb 13;21(2):30. doi: 10.1208/s12248-019-0303-4

Fig. 6. Effect of Bt2-cAMP on the degradation of cell surface hOAT3.

Fig. 6

(a) Top panel: COS-7 cells expressing hOAT3 were treated with the Bt2-cAMP (10μM). Cell surface hOAT3 degradation was then examined as described in the section of “Materials and Methods”, in conjunction with immunoblotting (IB) using anti-Myc antibody. Bottom panel: The identical blot as the top panel was re-probed with anti-E-cadherin antibody. E-cadherin is a cell membrane marker protein. (b) Densitometry analyses of results from Fig. 6a top panel along with other experiments. The amount of undegraded cell surface hOAT3 was expressed as % of total initial cell surface hOAT3 pool. Values are mean ± S.D. (n = 3). *P<0.05.