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. 2019 Jul 16;10:1630. doi: 10.3389/fmicb.2019.01630

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Copyright © 2019 Xie, Zhao, He, Qiu, Yuan, Ding, Liu, Jiang, Wang, Zhang, Zhang, Wang, Zhou, Zhang and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DNA prime-viral vectored vaccine boost strategies conferred protection against PR8/(H1N1) and H7N9 viruses in vaccinated mice. Mice were immunized following schedule outlined in Figure 2A, and 4 weeks later challenged with either 500 50% tissue culture-infective dose (TCID50) of A/PR8(H1N1) or 100 TCID50 A/Shanghai/4664T/2013(H7N9) influenza virus. After infection, mice (n = 5 in each group) were monitored daily for body weight (A,C) or survival (B,D). Five mice in each group were sacrificed on day 5 post infection to isolate lung tissues for RNA extraction, followed by RT-PCR quantification of viral RNA to determine the relative viral loads (E,F). The error bars represent the SDs. Two-way ANOVA test, Mantel–Cox log rank test and t-test were used to determine difference in weight loss, lethality, and viral load, respectively. ^∗∗p < 0.01; ^*p < 0.05.