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Expression of ATP1A3, gamma-glutamyl transferase, and megalin as a function of culture time and substrate stiffness. Expression levels of ATP1A3 (A), gamma-glutamyl transferase (GGT1) (B), and megalin (LRP2) (C) were determined by qPCR in primary HREC cultured on soft (0.5 kPa) and stiff (50 kPa) hydrogels. Cells were harvested at short (10 day) and long (4 weeks) time points.
