Figure 2.

Detection of viral genomes and transgene transcripts. Tissue samples were collected during necropsy. For each panel, perfused muscles correspond to biceps femoris, tibialis anterior, gastrocnemius medialis, and gastrocnemius lateralis of the injected limb; contralateral muscles correspond to the same muscles of the non-injected limb; and distant muscles correspond to biceps brachialis and brachioradialis of the two anterior limbs. D, H, L, and S indicate the diaphragm, heart, liver, and spleen, respectively. (A) Viral genomes were determined by quantitative polymerase chain reaction PCR (qPCR) and are expressed as viral genome per diploid genome (vg/dg). (B) Transcripts were detected by reverse transcription qPCR (RT-qPCR) and are expressed as relative quantity (RQ) to an endogenous gene. The gray areas correspond to the limit of quantification of PCR detection: 0.000809 vg/dg for (A) and RQ of 0.00304 for (B).