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. 2019 Jul 16;30(7):802–813. doi: 10.1089/hum.2018.234

Figure 3.

Figure 3.

No detection of anti-rtTA interferon gamma (IFN-γ) T-cell response at 5 years post injection. Peripheral blood mononuclear cells (PBMCs) were collected at euthanasia (5 years post injection), and secretion of IFN-γ by rtTA-stimulated PBMCs was evaluated by enzyme-linked immunospot assay. Cells were stimulated using an overlapping 15 per 10 amino acids peptide library covering the rtTA protein and divided in five pools (p1–p5). Negative controls (C–consisted of PBMCs cultured with medium alone, and positive control (C+) consisted of mitogenic Concanavalin A (Con A) stimulation. IFN-γ was measured as spot-forming cells (SFC). The threshold of positivity (dotted line) consisted of SFC >50 spots and three times the number of SFC obtained with negative control (C–). For positive responses, statistical analysis was performed using a DFR test. ***p ≤ 0.001; **p ≤ 0.01; *p ≤ 0.05. DFR, distribution free resampling.