Effect of gender on biochemical correction of skeletal muscle. Glycogen content for diaphragm (A) and quadriceps (B). Mean ± SEM is shown. Groups were as follows: untreated controls (GAA-KO mice: 4 males, 4 females); AAV only (GAA-KO mice: 5 males, 5 females); AAV+salmeterol (GAA-KO mice: 5 males, 5 females), and wildtype (WT; 5 males, 5 females). Two-way ANOVA with Bonferroni post-hoc analysis was performed; AAV and untreated groups were used as controls. Comparisons of male and female data are shown (C). Repeated t-tests were performed; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Mean ± standard deviation is shown.