Figure 6.

Vector administration decreased accumulated lysosomes and nuclei. Histology revealed that abnormalities were lower following vector administration in GAA-KO mice. Vacuolization score (A) and nuclei score (B) are shown for each treatment group. Number of mice analyzed: no treatment (3 males), vector (4 males), salmeterol (3 males), salmeterol + vector (3 males), and wildtype (4 males). Mean ± standard deviation is shown. *p < 0.05, **p < 0.01, and ***p < 0.001 for ANOVA with Bonferroni post-hoc analysis for multiple comparisons between all groups. (C) Representative photomicrographs of H&E sections of quadriceps muscle from each treatment group at 400 × showing the degree of vacuolar change within myofibers (thin arrows). Thick arrows depict nuclear profiles.