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. 2019 Jul 12;36(15):2308–2315. doi: 10.1089/neu.2018.6016

FIG. 4.

FIG. 4.

Representative hematoxylin and eosin (H&E) images from one control (A) and two spinal cord injury (B, C) mice reveal the different degrees of tissue loss at 3 days after injury. The signal intensity histogram of the raw H&E image (D) is shown as blue dots of the signal-intensity histogram (E). After deconvolution of raw signal intensity using multiple Gaussian functions, the highest signal portion of the image was identified (E; red Gaussian curve). Applying this component to the raw H&E image in (D), the extracellular space (F; red area) was automatically identified and quantified. Black scale bar in 20 × : 100 μm.