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Schematic representation of the hepatic differentiation protocol used for ORG HLC and representative pictures of the cells produced. All images were taken at the end of differentiation experiment. Image on the right panel was taken by allowing the HLC to explant from the 3D spheroids and attach, in 2D culture, and was only for photographic and morphological analysis. HLC, hepatocyte-like cell; ORG, organoid.
