FIG. 1.

HFHS causes fibrosis and cardiomyocyte hypertrophy after 4 months but not after 1 month on the diet. (A, B) Representative photomicrographs of Picro Sirius Red staining for cardiac fibrosis and quantification of cardiac fibrosis as measured by National Institutes of Health ImageJ software after (A) 1 month (mo.) and after (B) 4 months (mos.) on CD or HFHS diet. (C, D) Photomicrographs of left ventricular tissue sections stained by hematoxylin and eosin with quantification of myocyte cross-sectional area as measured by National Institutes of Health ImageJ after (C) 1 month and after (D) 4 months on CD or HFHS diet. Values are mean ± SEM; n = 3–4. *p < 0.05 versus CD-fed mice. CD, control diet; HFHS, high fat, high sucrose; SEM, standard error of the mean. Color images are available online.