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Compound 1a (100 μM) stabilizes Pin1 in HeLa cell lysates as determined by CETSA. (a) Pin1 content in the soluble fraction as revealed by Western blotting. (b) Plot of band intensity of Pin1 in the soluble fraction against temperature. The data were normalized to the Pin1 level of the control group at 45 °C and are expressed as the means ± SEM of three individual experiments.
