Figure 4.

Inhibition of Pin1–p65 interaction in cells: (a) 1a suppressed the Pin1–p65 interaction in HeLa cells as revealed by co-immunoprecipitation. HeLa cells were treated with the indicated concentration of 1a for 12 h. Protein lysates were incubated with antip65 and magnetic beads. The precipitated proteins were revealed by Western blotting with anti-Pin1 antibodies. 20% protein lysates were labeled as input were revealed by Western blotting with anti-p65 and anti-GAPDH antibodies. (b) 1a decreased the nuclear phospho-p65 level and had no effect on the cytoplasmic p65 level in HeLa cells.