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. 2019 Apr 18;47(13):e74. doi: 10.1093/nar/gkz267

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Sox9/PPAR-γ regulation by CRISPRai enhanced chondrogenesis and inhibited adipogenesis. (A–C) qRT-PCR analysis of Acan, Col2a1 and Co10a1. (D) Alcian blue staining. (E, F) qRT-PCR analysis of Fabp4 and C/ebpα. (G–I) Western blot and ensuing semi-quantitative analysis of FABP4 and C/EBPα. (J, K) Oil Red O staining and subsequent quantitative analysis. rBMSC in six-well plates were mock-transduced (Mock group) or co-transduced with Bac-aS-iP and Bac-Cre at MOI 300/100 (CRISPRai group). The cells were cultured in chondroinductive medium for 14 days for qRT-PCR (A–C) or for 21 days for Alcian blue staining (D). For adipogenesis, the cells were cultured in adipoinductive medium for 7 days for qRT-PCR (E, F) and Western blot (G–I) or for 14 days for Oil Red O staining (J, K). The data represent means ± SD of three independent culture experiments. ****P < 0.0001. ** P < 0.01. *P < 0.05.