Figure 3.

Laser-scanning confocal micrographs of an intact mesenteric artery from a Cre⁺/Dendra2⁺ mouse (left; A, C, E) and a Cre⁻/Dendra2⁺ (right; B, D, F) mouse. Nuclei were stained with DAPI (A, B). Green Dendra2 fluorescence was observed only in the Cre⁺/Dendra2⁺ artery (C), while no green fluorescence was observed in the artery from the Cre⁻/Dendra2⁺ mouse (D). Merged images indicate the green fluorescence of the Dendra2 protein co-localizes with EC nuclei (E), which run parallel to the lumen of the vessel. All images were taken at ×20 magnification and are maximum projection Z-stacks. Scale bar = 50 µm. Images were taken from three consecutive mice.