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. 2019 May 22;47(13):6796–6810. doi: 10.1093/nar/gkz431

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Expression of a phosphomimic CHK1 mutant restores wild-type levels of nascent ssDNA in WRN exonuclease-deficient cells. (A) WB analysis of FLAG-CHK1^WT and FLAG-CHK1^S317/345D expression in WS^E84A cells. (B) Evaluation of nascent ssDNA formation. Cells treated with 50nM CPT for 4 h were analyzed for the presence of nascent ssDNA by native anti-IdU detection. The graph shows the mean intensity of IdU fluorescence measured from two independent experiments (n = 200), data are presented as mean ± SE. Statistical analysis was performed by the Mann–Whitney test (*P < 0.05; ***P < 0.001; ****P < 0.0001). Representative images are shown.