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Optimization of buffer conditions and effects of Mg2+ (left panel) and Na+ (right panel) ions in Tris buffer. (A) R2FQ fluorescence alone. Fluorescence signal changes by (B) BePI (7.5 μM)-induced stabilization (ΔRFU = RFUwo/BePI - RFUw/BePI) and (C) MCRa2 (500 nM)-induced destabilization (ΔRFU = RFUw/MCRa2 – RFUwo/MCRa2).
