Table 2. . Effects of Common Cofactors and Inhibitors on the Enzymatic Activity.
| components | compoundsa | concn (mM) | relative activity (%) |
|---|---|---|---|
| Pro, Fe(II), 2-OG, VC | none | 0 | 100 |
| Pro, Fe(II), VC | none | 0 | 0 |
| Pro, 2-OG, VC | none | 0 | 14 |
| Pro, Fe(II), 2-OG | none | 0 | 69 |
| Pro, Fe(II), 2-OG, VC | EDTA | 4 | 3 |
| Pro, Fe(II), 2-OG, VC | 1,10-phenanthroline | 4 | 2 |
| Pro, Fe(II), 2-OG, VC | diethyl pyrocarbonate | 4 | 0 |
| Pro, Fe(II), 2-OG, VC | MgSO4 | 2 | 82 |
| Pro, Fe(II), 2-OG, VC | MnCl2 | 2 | 79 |
| Pro, Fe(II), 2-OG, VC | CoCl2 | 2 | 22 |
| Pro, Fe(II), 2-OG, VC | ZnSO4 | 2 | 12 |
| Pro, Fe(II), 2-OG, VC | CuSO4 | 2 | 14 |
| Pro, Fe(II), 2-OG, VC | NiSO4 | 2 | 7 |
a
Effects of chemicals and metal ions were assayed under standard assay conditions. Relative activity was calculated as percentages to the enzymatic activity under the following conditions: l-proline (Pro, 10 mM), 2-oxoglutarate (2-OG, 10 mM), ferrous sulfate (Fe(II), 1.5 mM), l-ascorbic acid (VC, 10 mM).