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B2C-induced cell death through the elevation of oxidative stress. Flow cytometric analysis of intracellular ROS in cancer cells treated with B2C. (a) SW620 cells, (b) DU145 cells, and (c) A549 cells. Cytotoxicity of B2C against (d) SW620 cells, (e) DU145 cells, and (f) A549 cells in the absence or presence of NAC. Values are mean ± SD (n = 4). ***p < 0.001 relative to a group of 0 μM; †††p < 0.001 relative to the same concentration of B2C + NAC (1 mM).
