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. Author manuscript; available in PMC: 2019 Sep 22.
Published in final edited form as: Methods Enzymol. 2018 Sep 22;612:225–249. doi: 10.1016/bs.mie.2018.08.031

Fig. 2.

Fig. 2

(A) Cell filtration set-up. (B) When liquid is almost finished filtering, immediately turn off vacuum, remove top and (C) scrape cells in one rapid motion. (D) A side view demonstrating the angle the reagent digger should be held at.