Fig. 5.

Ligation Size Selection Gel. (A) Products of the ligation reaction with the library and o199 control are run on a 10% TBE-Urea gel at 200 V for 50 min and stained with SYBR gold. The efficiency of ligation can be determined by comparing the intensity of unligated to ligated o199. Discrete bands in the footprint lane correspond to contaminants from rRNA fragments (B) Ligated products (35–65 nt) are excised and extracted. Note: This image was edited and lanes containing unrelated samples cut such that only pertinent information is displayed.