Ingredient	Final conc.	Stock conc.	Volume
Tris pH 8.0	20 mM	1 M	30 μl
NH4Cl*	100 mM	1 M	150 μl
MgCl2	10 mM	1 M	15 μl
Triton X-100*	0.4 %	25 %	24 μl
NP-40*	0.1 %	20 %	7.5 μl
Chloramphenicol**	1 mM	155 mM (50 mg/ml)	10 μl
RNase-free DNase	100 U/ml	10 U/μl	15 μl
DEPC H2O			1248.5 μl
Total volume			1500 μl

^*Prepare in advance and store at room temperature

^**Prepare in advance and store at −20 ºC