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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Nat Protoc. 2019 May 10;14(6):1863–1883. doi: 10.1038/s41596-019-0165-3
Component Amount (µl) Final concentration
Nuclease-free water 22
2x Q5 High-fidelity master mix 25 1X
5’ F primer (10 µM) 1 0.2 µM
3’ R primer (10 µM) 1 0.2 µM
Template DNA (50ng /µL) 1 1 ng/ µL
Total 50