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. 2019 Jul 16;10:1621. doi: 10.3389/fimmu.2019.01621

Figure 7.

Figure 7

Cardiac insulin sensitivity is preserved in obese NLRP3 deficient mice. WT, and Nlrp3−/− male mice were exposed to high fat diet (HFD; 60 cal% fat) or control diet for 52 weeks. (A) Left ventricle concentration of triglycerides [WT: Control, n = 10; HFD, n = 10, Nlrp3−/−: Control, n = 7; HFD, n = 7, and, Asc−/− (Pycard−/−): Control, n = 7; HFD, n = 7]. WT and Nlrp3−/− mice on control diet or HFD for 52 weeks were fasted for 4 h and received an i.p. injection of insulin (2 IU/kg). Mice were euthanized and after 10 min heart and liver were extirpated. Ratio of phosphorylated (Ser473) Akt to total Akt in mice on control diet or HFD in (B) left ventricle and (C) liver (WT: Control, n = 6; HFD, n = 4, Nlrp3−/−: Control, n = 4; HFD, n = 4) were determined by immunoblot analysis. Data are normalized to corresponding GAPDH. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 vs. control diet; #P < 0.05 vs. WT-HFD as determined by two-way ANOVA and Tukey's multiple comparisons test.