Table 5. Kinetic Parameters and Inhibition of DfrB1 and DfrB4 with Selected Inhibitors^a.

		DfrB1	DfrB4
kcat (s–1)		0.30 ± 0.04b	0.45 ± 0.03
KM (μM)	DHF	8.2 ± 0.11b	2.6 ± 1.0
	NADPH	1.6 ± 0.02b	2.8 ± 0.6
Ki (μM)c	TMP	(0.60 ± 0.14) × 103	(0.55 ± 0.10) × 103
	1d	4.0 ± 0.3	3.5 ± 1.1
	3	3.5 ± 1.7	3.9 ± 0.9
	33	7.4 ± 2.7	11 ± 1.3
	20b	2.6 ± 0.7	3.7 ± 0.7
	31	1.1 ± 0.3	1.9 ± 0.5

^aValues are given as the average ± standard deviation from the mean of at least duplicate of triplicate.

^bValues taken from Table 2 in ref (28).

^cK_i was calculated from IC₅₀ values (Table S8).

^dValue taken from Table 2 in ref (20).