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. 2019 Jul 16;10:1647. doi: 10.3389/fimmu.2019.01647

Figure 1.

Figure 1

IL-2, IL-15, IFN-β, and IFN-α could augment the nonspecific NK-ADCC function. (A) Representative flow plots of degranulation of NK cells in response to Ab-opsonized P815 cells (P815 + Ab), or medium or P815 cells alone after pre-incubation with different cytokines (50 ng/ml) for 12 h. (B) IL-2, IL-15, IFN-β, and IFN-α augmented CD107a production of activated NK cells during non-specific ADCC with stimulation of Ab-opsonized P815 cells (n = 9). (C) Representative flow plots of IFN-γ secretion of NK cells after pre-incubation with IL-2, IL-15, IFN-α, and IFN-β(50 ng/ml, 12 h). (D) IL-2, IL-15, IFN-β, and IFN-α increased IFN-γ secretion of NK cells during non-specific ADCC with stimulation of Ab-opsonized P815 cells(n = 10). (E) Effect of pre-incubation time of IL-2, IL-15, IFN-α, and IFN-β cytokines on NK-ADCC response. CD107a expression and IFN-γ secretion of NK cells were compared among samples pre-incubation with cytokines (50 ng/ml) for different hours (1, 6, 12, 18 h) with stimulation of Ab-opsonized P815 cells (n = 4). (F) Effect of cytokine concentrations on NK-ADCC response. CD107a expression and IFN-γ secretion of NK cells were compared among samples pre-incubation with different concentrations of IL-2, IL-15, IFN-α, and IFN-β cytokines (0, 10, 50, 100, 200 ng/ml) and stimulated with Ab-opsonized P815 cells for 12 h (n = 4). (G) Representative flow plots showing the lytic abilities of NK cells after pre-incubated with IL-2, IL-15, IFN-α, IFN-β (50 ng/ml, 12 h) and co-cultured with P815 cells or Ab-opsonized P815 cells for 6 h. Target P815 cells stained with PKH26+ CFSE−/low were indicated as lysed target cells. (H) Lysed rate of P815 target cells lysing by NK cells pre-incubated with IL-2, IL-15, IFN-α, or IFN-β (50 ng/ml, 12 h) and activated by Ab-opsonized cells subsequently (n = 6). Data is presented as mean ± SD. All P-values are two-tailed and considered to be significantly different with P < 0.05.